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Tsunami and Earthquake

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During catastrophes like tsunami and earthquake nerve injuries are so common and frequent since they combine crush syndrome with other associative lesion. Even wars create this risk and lead to treatment delays of wounded individuals with ballistic wounds. War victims are highly prone to emergency compared to vascular injuries.

This article sought to attempt transplanting adult neural stem cell (ANSCs) inside autologous venous graft using surgical transaction of nervous cruralis in an adult pig with a 30 mm long gap. The transplanted neural stem cell  call suspension was from an adult pig SVZ zone ; Subventicular zone labeled in Vitro with Lentivirally or BrdU  transferede proteins.  The Lesion-induced loss of leg extension on the thigh became ultimate in controls but was reversed by 45–90 days after neurosphere-filled vein grafting. The electromyography indicated that stimulodetection recovery in neurosphere-transplanted adult neural system but not in controls. On the other hand,  immunohistochemistry indicated that neurosphere-derived cells that survived inside the venous graft from 10 to 240 post-lesion days and all revealed a neuronal phenotype. Transportation of neural stem cells caused newly formed neurons to be distributed inside the autologous venous graft along the longitudinal nerve axis.. Furthermore, it was observed that ANSC transportation also increased the CNPase expression which indicates the activation of Schwann cells.

Intergration of new neurons inside the autologous lesion graft or activation of schwanncell has been considered as the genesis of the positive impact of most recent cell therapy protocol. The main advantage of recent procedures is attributed to neurosphere transplantation. LV.GFP has been proven to give an outstanding market for most grafted cells in long time studies and research.

Using large animals, particularly pigs in the above case allow repair and excellent study on long nerve gap and applying clinical conditions which are close to human beings. The study therefore provides hope and motivation in human anatomy, which leads to improvement in human clinics and surgery.

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